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E and F, Competition curves for steroid (E) and progestin (F) binding expressed as a percentage of maximum specific P4 binding, . Norp, Norprogesterone; Nandr, nandrolone; Ethist, ethisterone; Nore, norethisterone; Norg, norgestrel. The steroid binding characteristics of the recombinant human receptor (hu-mPRα) protein are similar to those of st-mPRα. Transfection of the MDA-MB-231 cells with hu-mPRα resulted in a 2. Progesterone ([ 3 H]-P4) was readily displaced from the receptor, and ligand association and dissociation were rapid, reaching 50% binding in approximately 5 min, which is typical of steroid membrane receptors ( Fig.
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